ISO INTERNATIONAL STANDARD 14371 First edition 2012-03-01 Water quality Determination of fresh water sediment toxicity to Heterocypris incongruens(Crustacea, Ostracoda) Qualité de I'eau-Détermination de la toxicite des sédiments d'eau douce envers Heterocypris incongruens (Crustacea, Ostracoda) Reference number ISO 14371:2012(E) LSO CopyihtInternatinal Organization for Standardization @ISO2012 ted without license from IHS Not for Resale ISO14371:2012(E) COPYRIGHTPROTECTEDDOCUMENT ISO2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either IsO at the address below or isO's memberbody in the country of therequester. ISO copyright office Casepostale 56·CH-1211Geneva20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2012 -All rights reserved No reproduction orr networking permitted without license from IHS Not for Resale ISO14371:2012(E) Contents Page Foreword ..iv Introduction 1 Scope 2 Normativereferences 3 Terms and definitions. 4 Principle. 5 Test environment. 2 6 Reagents,test organisms and media 2 7 Apparatusandmaterial 3 8 Special precautions for sampling,transportation, storage and treatment of the sedimentsamples 4 9 Procedure 4 9.1 Hatching of thecysts.. 9.2 Pre-feedingoftheostracodneonates 4 9.3 Lengthmeasurementoftheneonates 9.4 Additionofsedimentandalgalfoodtothetestcontainer 5 9.5 Transferoftheostracodneonatesintothetestcontainer 5 9.6 Incubationofthetest system. 9.7 Measurements. 6 10 Reference test 8 11 Testsonpurechemicalsorpreparations 10 12 Validity criteria 10 13 Test report AnnexA(informative)Culturing of Heterocypris incongruensfor cyst production AnnexB(informative)Precisiondata .13 Bibliography 15 ili ense from IHS Not for Resale